Dawn Heleos II Multi Angle Light Scattering

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Rates: $10 per injected sample

The DAWN® HELEOS® II is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. The DAWN offers the highest sensitivity, the widest range of molecular weight, size and concentration, and the largest selection of configurations and optional modules for enhanced capabiliites.

The DAWN is most often used in conjunction with Gel Permeation Chromatography (GPC-MALS) or Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.

The DAWN HELEOS II and its companion Optilab T-rEX dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.

SEC-MALS with a HELEOS II and Optilab characterizes conformation and branching of synthetic and natural polymers larger than 10 nm in rms radius Rg. The addition of a ViscoStar III differential viscometer creates a GPC triple-detection system can analyze molecular conformation and size below the rg limit of 10 nm and determine Mark-Houwink Sakurada coefficients.

The combination of a UV/Vis absorbance detector with a HELEOS and Optilab refractive index detector creates a UV-MALS-RI triple-detection system, for analysis of copolymers and protein conjugates (glycoproteins, PEGylated proteins, surfactant-bound membrane proteins, etc.). With the addition of a WyattQELS DLS module for size determination, protein conformation may be analyzed.

The DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size and second virial coefficient of unfractionated samples.

Included with each DAWN is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.

website: http://www.wyatt.com/products/instruments/dawn-heleos-ii-multi-angle-lig...

  • Mw from 200 Da to 1000 MDa
  • Sensitivity: 0.4 µg/mL BSA (66.4 kDa)
  • rg from 10 to 500 nm, up to 1000 nm with shape-specific models
  • Ultra-stable, high-power laser

Instrument Cluster includes:

Wyatt Dawn Helos-II

Wyatt Optilab T-Rex

Wyatt QELS

Waters HPLC Pump, including 1 spare Pump

For questions and Training, Contact Mike Estenson (michael-estenson@uiowa.edu)

 

ASTRA Software Guides:

ASTRA Quick Guide

Noise Assessment Guide

Calibration and Normalization Guide

 

 

 

 

 

 

 

 

 

 

Were you expecting to find a resource for this instrument here but didn’t find it?  Please contact michael-estenson@uiowa.edu about fixing that problem so that these valuable resources become easily accessible to you and other researchers!